- Our oldest machine is one that we built ourselves using the instructions and software kindly provided by Dr J. DeRisi at the time in the laboratory of Prof. P Brown. Some pictures of this machine are shown below. This machine uses the Arraymaker 2.6 software.
- The second machine is a Genomics Solutions: MicroGrid II. We use this machine for printing on both glass and nylon.
- Our last microarrayer is the biggest of the Arrayjet family. Unlike the two machines above this machine utilises an ink-jet type printing system rather than pins. Our machine is the Arrayjet UltraMarathon. With a capacity of 1000 slides this machine was the first of this type to be installed by Arrayjet.
We are using the GenePix 4000A and 4200A (four laser) scanners from Molecular Devices.
Commonly, though not exclusively, we utilise aldehyde slides and poly-L-lysine.
Pictures of the MArray Stanford design microarrayer.
The MArray DNA microarrayer is based on the
Stanford design and used a 16 and 32 tip head and printing tips from
MAJER
precision. It is controlled by the ArrayMaker software kindly provided by
Dr. J.DeRisi.
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A view of the array without the printing head installed. |
| A view of the 16 tip print head in action containing Die-Tech printing tips |
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Picture of the wash station containing ethanol, wash and drying stations. The ethanol wash station was an extra, we have not found a need to use it at the present time. |
| Picture of a Die-Tech printing tip which produces good features (spots) of approx. 100 micron. after some use |
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